Basic Vector Information
- Vector Name:
- pAP-ACN
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8196 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Wu S, Ying G, Wu Q, Capecchi MR.
pAP-ACN vector Map
pAP-ACN vector Sequence
LOCUS 40924_4569 8196 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pAP-ACN, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8196) AUTHORS Wu S, Ying G, Wu Q, Capecchi MR. TITLE A protocol for constructing gene targeting vectors: generating knockout mice for the cadherin family and beyond JOURNAL Nat Protoc 3 (6), 1056-1076 (2008) PUBMED 18546598 REFERENCE 2 (bases 1 to 8196) AUTHORS Wu S, Capecchi MR. TITLE Direct Submission JOURNAL Submitted (29-FEB-2008) Howard Hughes Medical Institute, University of Utah, 15N 2030E, Salt Lake City, UT 84112, USA REFERENCE 3 (bases 1 to 8196) TITLE Direct Submission REFERENCE 4 (bases 1 to 8196) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc"; date: "2008"; volume: "3"; issue: "6"; pages: "1056-1076" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (29-FEB-2008) Howard Hughes Medical Institute, University of Utah, 15N 2030E, Salt Lake City, UT 84112, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..8196 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 34..52 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 84..1586 /label=SEAP /note="secreted alkaline phosphatase from human placenta" polyA_signal 1705..2099 /label=beta-globin poly(A) /note="human beta-globin polyadenylation signal" protein_bind 2111..2144 /label=loxP /note="Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (Shaw et al., 2021)." polyA_signal 2171..2219 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" regulatory complement(2171..2218) /label=HSV thymidine kinase polyA signal /note="HSV thymidine kinase polyA signal" /regulatory_class="polyA_signal_sequence" CDS complement(2229..3029) /label=NeoR/KanR /note="aminoglycoside phosphotransferase from Tn5" regulatory complement(3046..3752) /label=RNA polymerase II large subunit promoter /note="RNA polymerase II large subunit promoter" /regulatory_class="promoter" polyA_signal complement(3768..3816) /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" intron complement(4166..4231) /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS complement(5057..5077) /label=SV40 NLS /note="nuclear localization signal of SV40 (simian virus 40) large T antigen" CDS complement(5101..5112) /label=WELQut site /note="WELQut protease recognition and cleavage site" misc_recomb 5787..5820 /label=loxP site /note="loxP site" protein_bind complement(5787..5820) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." promoter complement(5884..5902) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" promoter 6248..6352 /label=AmpR promoter CDS 6353..7210 /label=AmpR /note="beta-lactamase" rep_origin 7384..7972 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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