pAMG258 vector (V009509)

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LOCUS       V009509                 8662 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009509
VERSION     V009509
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8662)
  AUTHORS   Olson DG.
  TITLE     Transformation of Clostridium thermocellum by electroporation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8662)
  AUTHORS   Olson DG.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-OCT-2011) Thayer School of Engineering, Dartmouth
            College, 8000 Cummings, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 8662)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8662)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-OCT-2011) Thayer School of Engineering, Dartmouth College, 8000
            Cummings, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    11..514
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     regulatory      692..1339
                     /gene="GapDH"
                     /regulatory_class="promoter"
     gene            692..1339
                     /gene="GapDH"
                     /label="GapDH"
     CDS             1340..1987
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             2008..2553
                     /codon_start=1
                     /gene="hpt"
                     /product="hypoxanthine phosphoribosyltransferase"
                     /label="hpt"
                     /protein_id="AFC37773.1"
                     /translation="MENLSKDIDEILITEEELKEKIKELGRQITKDYKGKNLMLVGVLK
                     GALMFMADLSRHIDLPLSLDFMAVSSYGSSTHSSGIVKIIKDLDISIEGKDVLIVEDII
                     DSGLTLSYLRETLLGRKPKSLKICTILDKPERREASVKVDYVGFKIPDKFVVGYGLDFD
                     EKYRNLPFIGVLKPEMYS"
     gene            2008..2553
                     /gene="hpt"
                     /label="hpt"
     terminator      2663..2910
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(3158..3746)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3833..4411)
                     /codon_start=1
                     /gene="tdk"
                     /product="thymidine kinase"
                     /label="tdk"
                     /protein_id="AFC37774.1"
                     /translation="MYGPKDHGYIEVVTGPMFSGKSEELIRRIKRAKIARQKVQVFKPA
                     IDDRYSIDKVVSHNGDNMHAIAIVKASDILAYAEEDTDVFAIDEVQFFDSEIVDIVKEI
                     ADSGKRVICAGLDMDFRGEPFGPTPELMAIAEFVDKLTAICMKCGNPATRTQRLINGKP
                     ANYDDPIIMVGAKESYEARCRKCHEVPRT"
     gene            complement(3833..4411)
                     /gene="tdk"
                     /label="tdk"
     regulatory      complement(4412..5032)
                     /gene="Cbp"
                     /regulatory_class="promoter"
     gene            complement(4412..5032)
                     /gene="Cbp"
                     /label="Cbp"
     CDS             complement(5111..6112)
                     /label="repB"
                     /note="RepB replication protein"
     rep_origin      complement(6113..6567)
                     /direction=LEFT
                     /label="cth ori"
                     /note="cth ori"
     CDS             complement(6673..7530)
                     /label="AmpR"
                     /note="beta-lactamase"
     CDS             complement(7629..8429)
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(8430..8645)
                     /label="URA3 promoter"