pAM656 vector (V009512)

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LOCUS       40924_4409        5255 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Saccharomyces cerevisiae vector pAM656, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Agaphonov M, Alexandrov A.
  TITLE     Self-excising integrative yeast plasmid vectors containing an 
            intronated recombinase gene
  JOURNAL   FEMS Yeast Res. (2014) In press
  PUBMED    25124534
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Agaphonov M, Alexandrov A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2014) A.N. Bach Institute of Biochemistry, Russian
            Academy of Science, Moscow 119071, Russian Federation
REFERENCE   3  (bases 1 to 5255)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5255)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-MAY-2014) A.N. Bach Institute of Biochemistry, Russian Academy 
            of Science, Moscow 119071, Russian Federation"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(22..38)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    51..84
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(109..550)
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     intron          684..734
     CDS             738..1721
                     /label=Cre
                     /note="site-specific recombinase"
     rep_origin      complement(1797..2385)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2559..3416)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3417..3521)
                     /label=AmpR promoter
     promoter        3627..4029
                     /label=LEU2 promoter
     CDS             4030..5121
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     misc_recomb     complement(5195..5228)
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(5195..5228)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."