pAM1 vector (V009520)

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LOCUS       V009520                 6245 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009520
VERSION     V009520
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6245)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6245)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6245)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(502..1233)
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     CDS             1698..1982
                     /codon_start=1
                     /note="unnamed protein product; copG-like"
                     /protein_id="SJL86728.1"
                     /translation="MSDFSERDRELLAMFGMGEDQVRDDVERMESETAGHGITGPVHYA
                     LHMLPEHGEEMVSMTVKLPKSQLEKVTETARRYHISRSEYVRRQLAGAV"
     CDS             complement(2174..2464)
                     /codon_start=1
                     /note="unnamed protein product; orfX-like"
                     /protein_id="SJL86729.1"
                     /translation="MTIQTIRKKRPLPAKELAAMYDVSVRTIQRWASQTRKDWIDEQAT
                     LRESIRAYHDDEGHTWPQTAEHFGMSQDAVRSRCYRARKERAAEAKAARPE"
     CDS             complement(2461..3408)
                     /codon_start=1
                     /note="unnamed protein product; repB"
                     /protein_id="SJL86730.1"
                     /translation="MSDEYSQPTLELSRTFEGWWLPRRPLCCDDDYSQLVRRSRTDALR
                     CKHIEANPSALVNTIVVDIDDANAKAMALWGHRGMLPNWIAENPANGHAHAGWVLTYPV
                     PRTDMARLKPLKLLHAVTEGLRRSVDGDEGYSGLLMKNPLSDAWDSDLCREDTYDLPDL
                     VAALEEHGDMPPKSWTRTKRAREVGVGRNCTLFDEARTLAYRQVRRLPDRTPASSDLLR
                     EYVRRTCHEINASFPDPLPVREVNDTAKSIHKWITTRSRMWRDGAVANAATFVAIQSAR
                     GRKGGRGNKRDKQGNVQNAFKQKAELFGKEMMGQ"
     primer_bind     complement(4024..4040)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4048..4064)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4072..4102)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4117..4138)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4426..5014)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5188..6045)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6046..6150)
                     /label="AmpR promoter"