pALE2 vector (V009528)

LOCUS       V009528                 7366 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009528
VERSION     V009528
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7366)
  AUTHORS   Fortune SM, Jaeger A, Sarracino DA, Chase MR, Sassetti CM, Sherman
            DR, Bloom BR, Rubin EJ.
  TITLE     Mutually dependent secretion of proteins required for mycobacterial
            virulence
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 102 (30), 10676-10681 (2005)
   PUBMED   16030141
REFERENCE   2  (bases 1 to 7366)
  AUTHORS   Fortune SM, Jaeger A, Sherman DR, Rubin EJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUN-2005) IID, Harvard School of Public Health, 200
            Longwood Ave., Boston, MA 02115, USA
REFERENCE   3  (bases 1 to 7366)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7366)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2005"; volume: "102"; issue: "30"; pages:
            "10676-10681"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-JUN-2005) IID, Harvard School of Public Health, 200 Longwood
            Ave., Boston, MA 02115, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      52..138
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      230..257
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        303..333
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(339..358)
                     /label="lac operator (symmetric)"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The
                     symmetric lac operator was optimized for tight binding of
                     lac repressor."
     CDS             387..1562
                     /gene="espA"
                     /label="ESX-1 secretion-associated protein EspA"
                     /note="ESX-1 secretion-associated protein EspA from
                     Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
                     Accession#: P9WJE1"
     CDS             1569..1586
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             1593..1622
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     terminator      1666..1713
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      complement(1917..2505)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2827..3483)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3484..3584)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             complement(3582..4547)
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     CDS             complement(5743..6855)
                     /gene="33"
                     /label="Integrase"
                     /note="Integrase from Mycobacterium phage L5. Accession#:
                     P22884"