pAL004(Ubi-nos)mDII vector (V009531)

LOCUS       40924_4309       12582 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAL004(Ubi-nos)mDII, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12582)
  AUTHORS   Mir R, Aranda LZ, Baiocchi T, Luo A, Sylvester A, Rasmussen C.
  TITLE     DII-based auxin reporter in maize reveals low auxin signaling during
            telophase and early G1
  JOURNAL   Plant Physiol. (2016) In press
  PUBMED    27881728
REFERENCE   2  (bases 1 to 12582)
  AUTHORS   Rasmussen CG.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2016) Botany and Plant Sciences, University of 
            California, Riverside, 900 E. University Ave., Riverside, CA 92521, 
            USA
REFERENCE   3  (bases 1 to 12582)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12582)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-DEC-2016) Botany and Plant Sciences, University of California, 
            Riverside, 900 E. University Ave., Riverside, CA 92521, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(115..139)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    297..318
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        333..363
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    371..387
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     395..411
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        436..2432
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     misc_feature    2455..2556
                     /label=derived from mDegron
                     /note="derived from mDegron"
     protein_bind    complement(2570..2594)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2608..3321
                     /label=Venus
                     /note="yellow fluorescent protein (YFP) with fast and
                     efficient maturation (Nagai et al., 2002)"
     misc_feature    3337..3576
                     /label=derived from N7-NLS sequence
                     /note="derived from N7-NLS sequence"
     terminator      3599..3851
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        3959..4630
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     regulatory      4644..4770
                     /label=TEV
                     /note="TEV"
                     /regulatory_class="enhancer"
     CDS             4782..5330
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     primer_bind     complement(5901..5917)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(6132..6156)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6680..7468
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      7717..8305
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8491..8631)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8975..9169)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9238..10308)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10740..11366)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"