pAK405 vector (V009536)

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LOCUS       V009536                 3937 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009536
VERSION     V009536
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A.
  TITLE     Markerless gene deletion system for sphingomonads
  JOURNAL   Appl. Environ. Microbiol. 78 (10), 3774-3777 (2012)
   PUBMED   22427496
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2012) Department of Biology, Institute of
            Microbiology, ETH Zurich, Wolfgang-Pauli-Str. 10, Zurich, Zurich
            8093, Switzerland
REFERENCE   3  (bases 1 to 3937)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3937)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "10";
            pages: "3774-3777"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-JAN-2012) Department of Biology, Institute of Microbiology, ETH
            Zurich, Wolfgang-Pauli-Str. 10, Zurich, Zurich 8093, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             346..1137
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     regulatory      1167..1207
                     /gene="Psyn2"
                     /label="synthetic bacterial promoter"
                     /note="synthetic bacterial promoter"
                     /regulatory_class="promoter"
     gene            1167..1207
                     /gene="Psyn2"
                     /label="Psyn2"
     regulatory      1221..1229
                     /gene="rpsL1"
                     /regulatory_class="ribosome_binding_site"
     CDS             1234..1602
                     /gene="rpsL"
                     /label="Small ribosomal subunit protein uS12"
                     /note="Small ribosomal subunit protein uS12 from
                     Rhizorhabdus wittichii (strain DSM 6014 / CCUG 31198 / JCM
                     15750 / NBRC 105917 / EY 4224 / RW1). Accession#: A5V607"
     oriT            complement(1625..1734)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     promoter        complement(1969..2059)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     promoter        complement(2253..2343)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      2703..3291
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    3579..3600
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3615..3645
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    3653..3669
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3677..3693
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    3702..3758
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(3762..3778)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"