pAJM.771 vector (V009544)

LOCUS       V009544                 4184 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009544
VERSION     V009544
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Meyer AJ, Segall-Shapiro TH, Voigt CA.
  TITLE     Marionette: E. coli containing 12 highly-optimized small molecule
            sensors
  JOURNAL   bioRxivorg 285866, 1-26 (2018)
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Meyer AJ, Segall-Shapiro TH, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2018) Synthetic Biology Center, Department of
            Biological Engineering, Massachusetts Institute of Technology, 77
            Massachusetts Avenue, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 4184)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4184)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxivorg
            285866, 1-26 (2018)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-MAR-2018) Synthetic Biology Center, Department of Biological
            Engineering, Massachusetts Institute of Technology, 77 Massachusetts
            Avenue, Cambridge, MA 02139, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..53
                     /label="L3S3P21"
                     /note="L3S3P21"
                     /regulatory_class="terminator"
     regulatory      73..188
                     /label="PSalTTC"
                     /note="PSalTTC"
                     /regulatory_class="promoter"
     misc_feature    110..140
                     /label="NahO operator"
                     /note="NahO operator"
     regulatory      154..159
                     /regulatory_class="minus_35_signal"
     regulatory      176..181
                     /regulatory_class="minus_10_signal"
     misc_RNA        190..240
                     /label="sTRSV HHRz"
                     /note="hammerhead ribozyme from the tobacco ringspot virus
                     satellite RNA (Khvorova et al., 2003)"
     regulatory      272..283
                     /label="B0064"
                     /note="B0064"
                     /regulatory_class="ribosome_binding_site"
     CDS             290..1006
                     /label="EYFP"
                     /note="enhanced YFP"
     regulatory      1010..1069
                     /label="L3S2P21"
                     /note="L3S2P21"
                     /regulatory_class="terminator"
     regulatory      1074..1127
                     /label="PLacIQ"
                     /note="PLacIQ"
                     /regulatory_class="promoter"
     regulatory      1092..1097
                     /regulatory_class="minus_35_signal"
     regulatory      1115..1120
                     /regulatory_class="minus_10_signal"
     regulatory      1128..1156
                     /label="nah2"
                     /note="nah2"
                     /regulatory_class="ribosome_binding_site"
     CDS             1157..2056
                     /gene="nahR"
                     /label="HTH-type transcriptional activator NahR"
                     /note="HTH-type transcriptional activator NahR from
                     Pseudomonas putida. Accession#: P10183"
     regulatory      2069..2323
                     /label="induction operon terminator region"
                     /note="induction operon terminator region"
                     /regulatory_class="terminator"
     rep_origin      complement(2635..3180)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(3272..4084)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"