pAJM.677 vector (V009548)

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LOCUS       V009548                 5793 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009548
VERSION     V009548
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Meyer AJ, Segall-Shapiro TH, Voigt CA.
  TITLE     Marionette: E. coli containing 12 highly-optimized small molecule
            sensors
  JOURNAL   bioRxivorg 285866, 1-26 (2018)
REFERENCE   2  (bases 1 to 5793)
  AUTHORS   Meyer AJ, Segall-Shapiro TH, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2018) Synthetic Biology Center, Department of
            Biological Engineering, Massachusetts Institute of Technology, 77
            Massachusetts Avenue, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 5793)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5793)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxivorg
            285866, 1-26 (2018)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-MAR-2018) Synthetic Biology Center, Department of Biological
            Engineering, Massachusetts Institute of Technology, 77 Massachusetts
            Avenue, Cambridge, MA 02139, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..53
                     /label="L3S3P21"
                     /note="L3S3P21"
                     /regulatory_class="terminator"
     promoter        72..356
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     misc_RNA        363..413
                     /label="sTRSV HHRz"
                     /note="hammerhead ribozyme from the tobacco ringspot virus
                     satellite RNA (Khvorova et al., 2003)"
     regulatory      445..456
                     /label="B0064"
                     /note="B0064"
                     /regulatory_class="ribosome_binding_site"
     CDS             463..1179
                     /label="EYFP"
                     /note="enhanced YFP"
     regulatory      1183..1243
                     /label="L3S2P21"
                     /note="L3S2P21"
                     /regulatory_class="terminator"
     regulatory      1248..1301
                     /label="PLacIQ"
                     /note="PLacIQ"
                     /regulatory_class="promoter"
     regulatory      1266..1271
                     /regulatory_class="minus_35_signal"
     regulatory      1289..1294
                     /regulatory_class="minus_10_signal"
     regulatory      1302..1329
                     /label="ara1"
                     /note="ara1"
                     /regulatory_class="ribosome_binding_site"
     CDS             1330..2205
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     regulatory      2209..2249
                     /label="e1"
                     /note="e1"
                     /regulatory_class="ribosome_binding_site"
     CDS             2250..3665
                     /gene="araE"
                     /label="Arabinose-proton symporter"
                     /note="Arabinose-proton symporter from Escherichia coli
                     (strain K12). Accession#: P0AE24"
     regulatory      3678..3932
                     /label="induction operon terminator region"
                     /note="induction operon terminator region"
                     /regulatory_class="terminator"
     rep_origin      complement(4244..4789)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(4881..5693)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"