pAGW641 vector (V009563)

LOCUS       40924_4145       10468 bp DNA     circular SYN 17-DEC-2018
DEFINITION  PtGAUT12 RNAi binary vector pAGW641, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10468)
  AUTHORS   Biswal AK, Hao Z, Pattathil S, Yang X, Winkeler K, Collins C, 
            Mohanty SS, Richardson EA, Gelineo-Albersheim I, Hunt K, Ryno D, 
            Sykes RW, Turner GB, Ziebell A, Gjersing E, Lukowitz W, Davis MF, 
            Decker SR, Hahn MG, Mohnen D.
  TITLE     Downregulation of GAUT12 in Populus deltoides by RNA silencing 
            results in reduced recalcitrance, increased growth and reduced xylan
            and pectin in a woody biofuel feedstock
  JOURNAL   Biotechnol Biofuels 8, 41 (2015)
  PUBMED    25802552
REFERENCE   2  (bases 1 to 10468)
  AUTHORS   Rottmann WH, Winkeler KA, Biswal AK, Yang X.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2014) ArborGen Inc., 2011 Broadbank Ct., 
            Ridgeville, SC 29472, USA
REFERENCE   3  (bases 1 to 10468)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10468)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Biofuels 8, 41 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-DEC-2014) ArborGen Inc., 2011 Broadbank Ct., Ridgeville, SC 
            29472, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     On Apr 14, 2015 this sequence version replaced KP259612.1.
FEATURES             Location/Qualifiers
     source          1..10468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             802..1593
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             1895..3040
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     misc_feature    3495..3519
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     3857..3873
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(3875..4095)
                     /gene="nos"
                     /label=nopaline synthase (nos) terminator
                     /note="nopaline synthase (nos) terminator"
                     /regulatory_class="terminator"
     terminator      3886..4138
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4144..4935)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(4936..6262)
                     /gene="nos"
                     /note="Arabidopsis thaliana UBIQUITIN10 (At4g05320)
                     promoter plus 5'-UTR"
                     /regulatory_class="promoter"
     terminator      complement(6300..6552)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    6610..6634
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6661..6828
                     /label=fragment of PtGAUT12
                     /note="fragment of PtGAUT12"
     protein_bind    complement(6851..6875)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          complement(6904..8245)
                     /label=chsA intron
                     /note="chalcone synthase A intron from Petunia hybrida"
     protein_bind    8262..8286
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    complement(8309..8476)
                     /label=fragment of PtGAUT12
                     /note="fragment of PtGAUT12"
     protein_bind    complement(8503..8527)
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     rep_origin      10000..10468
                     /label=oriV
                     /note="incP origin of replication"