pAGW633 vector (V009564)

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LOCUS       40924_4140       10326 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pAGW633, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10326)
  AUTHORS   Biswal AK, Atmodjo MA, Li M, Yoo CG, Pu Y, Lee Y-C., Zhang J-Y., 
            Ramanna H, Bray AL, King ZR, LaFayette PR, Mohanty SS, Ryno D, Yee 
            K, Thompson OA, Rodriguez M Jr., Winkeler KA, Collins C, Yang X, Tan
            L, Sykes RW, Gjersing EL, Ziebell A, Turner GB, Decker SR, Parrott 
            WA, Udvardi MK, Mielenz J, Davis MF, Nelson RS, Ragauskas AJ, Mohnen
            D.
  TITLE     Targeted modification of pectin biosynthesis to improve growth and 
            deconstruction of woody and grass bioenergy feedstocks
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10326)
  AUTHORS   Rottmann WH, Winkeler KA, Biswal AK, Yang X.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2016) Biochemistry and Molecular Biology, Complex 
            Carbohydrate Research Center, The University of Georgia, 315 
            Riverbend Rd, Athens, GA 30602, USA
REFERENCE   3  (bases 1 to 10326)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10326)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-AUG-2016) Biochemistry and Molecular Biology, Complex 
            Carbohydrate Research Center, The University of Georgia, 315 
            Riverbend Rd, Athens, GA 30602, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             801..1592
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             1894..3039
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     misc_feature    3399..3423
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     3761..3777
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(3779..3999)
                     /gene="nos"
                     /label=nopaline synthase (nos) terminator
                     /note="nopaline synthase (nos) terminator"
                     /regulatory_class="terminator"
     terminator      3790..4042
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4048..4839)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    complement(4840..6166)
                     /gene="nos"
                     /note="Arabidopsis thaliana ubiquitni 10 (At4g05320)
                     promoter and 5' UTR"
     terminator      complement(6204..6456)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    6514..6538
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    complement(6565..6714)
                     /label=fragment of PtGAUT4
                     /note="fragment of PtGAUT4"
     protein_bind    complement(6732..6756)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          complement(6785..8126)
                     /label=chsA intron
                     /note="chalcone synthase A intron from Petunia hybrida"
     protein_bind    8143..8167
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    complement(8185..8334)
                     /label=fragment of PtGAUT4
                     /note="fragment of PtGAUT4"
     protein_bind    complement(8361..8385)
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     rep_origin      10000..10326
                     /label=oriV
                     /note="incP origin of replication"