pAGW570 vector (V009565)

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LOCUS       40924_4135        9773 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pAGW570, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9773)
  AUTHORS   Biswal AK, Atmodjo MA, Pattathil S, Amos RA, Yang X, Winkeler K, 
            Collins C, Mohanty SS, Ryno D, Tan L, Gelineo-Albersheim I, Hunt K, 
            Sykes RW, Turner GB, Ziebell A, Davis MF, Decker SR, Hahn MG, Mohnen
            D.
  TITLE     Increased xylan production by overexpression of 
            GalactUronosylTransferase12 (GAUT12) results in increased 
            recalcitrance and decreased growth in Populus woody biofuel 
            feedstock
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9773)
  AUTHORS   Rottmann W, Winkeler K, Biswal AK, Yang X.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2017) Biochemistry and Molecular Biology; Complex 
            Carbohydrate Research Center, The University of Georgia, 315 
            Riverbend Rd, Athens, GA 30602, USA
REFERENCE   3  (bases 1 to 9773)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9773)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-JUN-2017) Biochemistry and Molecular Biology; Complex 
            Carbohydrate Research Center, The University of Georgia, 315 
            Riverbend Rd, Athens, GA 30602, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      104..634
                     /label=oriV
                     /note="incP origin of replication"
     CDS             907..1698
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             2000..3145
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     misc_feature    3505..3529
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     3867..3883
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(3885..4105)
                     /label=nopaline synthase (nos) terminator
                     /note="nopaline synthase (nos) terminator"
                     /regulatory_class="terminator"
     terminator      3896..4148
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4154..4945)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(4946..6272)
                     /note="Arabidopsis thaliana UBIQUITIN10 (At4g05320)
                     promoter plus 5'-UTR"
                     /regulatory_class="promoter"
     terminator      complement(6310..6562)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    6597..6621
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(6646..8247)
                     /codon_start=1
                     /product="galacturonosyltransferase 12"
                     /label=galacturonosyltransferase 12
                     /note="PtGAUT12.1 (Potri.001G416800); GAUT12"
                     /protein_id="AUG68175.1"
                     /translation="MQLHISPSLRHVTVFPGKGVREFIKVRVGARRVSYRMLFYSLLFF
                     TFLLRFVFVLSTVDSIDGETKCSTLGCLGKRLGPRILGRRLDSAVPEVMFQVLEQPLGN
                     DELKGRSDIPQTLEEFMDEVKNTRLDAKTFALKLREMVTLLEQRTRNAKIQEYLYRHVA
                     SSSIPKQLHCLALRLASEHSTNAAARLQLPLPELVPALVDNTYFHFVLASDNVLAAAVV
                     ANSLVQNALRPQKFVLHIITDRKTYSPMQAWFSLHPLAPAIIEVKALHHFDWFAKGKVP
                     VMEAMEKDQRVRSQFRGGSSAIVANNTEKPHIIAAKLQTLSPKYNSVMNHIRIHLPELF
                     PSLNKVVFLDDDIVVQSDLSPLWDIDMNGKVNGAVETCRGEDKFVMSKKLKSYLNFSHP
                     LISENFKPNECAWAYGMNIFDLEAWRKTNISTTYHHWVEENLKSDLSLWQLGTLPPGLI
                     AFHGHVHVIDPFWHMLGLGYQENTSLADAETAGVIHFNGRAKPWLDIAFPQLRPLWAKY
                     INFSDKFIKGCHIRPS"
     protein_bind    complement(8274..8298)
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      complement(8320..9672)
                     /note="Arabidopsis thaliana UBIQUITIN3 (At5g03240) promoter
                     (- intron)"
                     /regulatory_class="promoter"
     primer_bind     complement(9676..9692)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    9745..9769
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"