pAGSM552 vector (V009566)

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LOCUS       40924_4130       12660 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary RNAi Gateway destination vector pAGSM552, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12660)
  AUTHORS   Rottmann WH, Miller SA.
  TITLE     Plasmids used by BioEnergy Science Center (BESC) for Populus 
            transformation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12660)
  AUTHORS   Rottmann WH, Miller SA.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2014) ArborGen Inc., 2011 Broadbank Ct., 
            Ridgeville, SC 29472, USA
REFERENCE   3  (bases 1 to 12660)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12660)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-DEC-2014) ArborGen Inc., 2011 Broadbank Ct., Ridgeville, SC 
            29472, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     On Apr 14, 2015 this sequence version replaced KP259613.1.
FEATURES             Location/Qualifiers
     source          1..12660
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             802..1593
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             1895..3040
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     misc_feature    3495..3519
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     3857..3873
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(3875..4095)
                     /gene="nos"
                     /label=nopaline synthase (nos) terminator
                     /note="nopaline synthase (nos) terminator"
                     /regulatory_class="terminator"
     terminator      3886..4138
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4144..4935)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(4936..6262)
                     /gene="nos"
                     /note="Arabidopsis thaliana UBIQUITIN10 (At4g05320)
                     promoter plus 5'-UTR"
                     /regulatory_class="promoter"
     intron          complement(4936..5239)
                     /gene="nos"
     terminator      complement(6300..6552)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    6610..6734
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        6759..6789
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             complement(6855..7157)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(7505..7629)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          complement(7657..8998)
                     /label=chsA intron
                     /note="chalcone synthase A intron from Petunia hybrida"
     protein_bind    9015..9139
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(9183..9485)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     rep_origin      10000..10529
                     /label=oriV
                     /note="incP origin of replication"
     promoter        complement(10540..10570)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(10595..10719)
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      complement(10753..12481)
                     /note="Arabidopsis thaliana UBIQUITIN3 (At5g03240) promoter
                     plus 5'-UTR"
                     /regulatory_class="promoter"
     intron          complement(10753..11127)
     misc_feature    12527..12551
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"