Basic Vector Information
- Vector Name:
- pAgc-bgyg
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10385 bp
- Type:
- Reporter vector
- Replication origin:
- ori
- Source/Author:
- Ikeda Y, Ito K, Izumi Y, Shinomura T.
pAgc-bgyg vector Map
pAgc-bgyg vector Sequence
LOCUS 40924_4120 10385 bp DNA circular SYN 17-DEC-2018 DEFINITION Reporter vector pAgc-bgyg DNA, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10385) AUTHORS Ikeda Y, Ito K, Izumi Y, Shinomura T. TITLE A candidate enhancer element responsible for high-level expression of the aggrecan gene in chondrocytes JOURNAL J. Biochem. 156 (1), 21-28 (2014) PUBMED 24554731 REFERENCE 2 (bases 1 to 10385) AUTHORS Ikeda Y, Shinomura T. TITLE Direct Submission JOURNAL Submitted (01-AUG-2013) Contact:Yuichi Ikeda Tokyo Medical and Dental University, Tissue Regeneration; 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan URL :http://www.tmd.ac.jp/ REFERENCE 3 (bases 1 to 10385) TITLE Direct Submission REFERENCE 4 (bases 1 to 10385) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J. Biochem."; date: "2014"; volume: "156"; issue: "1"; pages: "21-28" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (01-AUG-2013) Contact:Yuichi Ikeda Tokyo Medical and Dental University, Tissue Regeneration; 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan URL :http://www.tmd.ac.jp/" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..10385 /mol_type="other DNA" /organism="synthetic DNA construct" regulatory 17..550 /gene="bgyg" /label=rat Agc1 promoter sequence /note="rat Agc1 promoter sequence" /regulatory_class="promoter" exon 551..931 /gene="bgyg" /note="rat Agc1 exon 1 sequence" intron 928..1990 /gene="bgyg" CDS 2172..5213 /label=lacZ /note="beta-galactosidase" CDS 5229..6263 /label=HygR /note="hygromycin B phosphotransferase" promoter complement(7254..7272) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(7290..7306) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 7314..7330 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7338..7368) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(7383..7404) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(7692..8280) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(8454..9311) /label=AmpR /note="beta-lactamase" promoter complement(9312..9416) /label=AmpR promoter rep_origin 9750..10205 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 10346..10362 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants"
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