pADNpcDNA3.1 KanR vector (V009575)

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LOCUS       40924_4075       10000 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pADNpcDNA3.1 KanR, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10000)
  AUTHORS   Ambati S, Yu P, McKinney EC, Kandasamy MK, Hartzell D, Baile CA, 
            Meagher RB.
  TITLE     Adipocyte nuclei captured from VAT and SAT
  JOURNAL   BMC Obes 3, 35 (2016)
  PUBMED    27462403
REFERENCE   2  (bases 1 to 10000)
  AUTHORS   Ambati S, Yu P, McKinney EC, Kandasamy MK, Hartzell D, Baile CA, 
            Meagher RB.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAY-2016) Genetics, University of Georgia, 120 East 
            Green Street, Athens, GA 30602, USA
REFERENCE   3  (bases 1 to 10000)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10000)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Obes 3,
            35 (2016)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-MAY-2016) Genetics, University of Georgia, 120 East Green 
            Street, Athens, GA 30602, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      37..5486
                     /label=ADNp*
                     /note="ADNp*"
                     /regulatory_class="promoter"
     misc_feature    2820..2825
                     /label=AgeI site
                     /note="AgeI site"
     misc_feature    5487..5548
                     /label=multiple cloning site
                     /note="multiple cloning site"
     polyA_signal    5580..5804
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5850..6278
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6292..6621
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             6688..7479
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    7656..7789
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7826..7842)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7850..7866
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7874..7904)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7919..7940)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8248..8833)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9007..9864)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9865..9969)
                     /label=AmpR promoter