Basic Vector Information
- Vector Name:
- pADIS1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5106 bp
- Type:
- Cloning vector
- Replication origin:
- oriV
- Source/Author:
- Li Y, Rosso MG, Strizhov N, Viehoever P, Weisshaar B.
pADIS1 vector Map
pADIS1 vector Sequence
LOCUS V009576 5106 bp DNA circular SYN 17-DEC-2018 DEFINITION Exported. ACCESSION V009576 VERSION V009576 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 5106) AUTHORS Li Y, Rosso MG, Strizhov N, Viehoever P, Weisshaar B. TITLE GABI-Kat SimpleSearch: a flanking sequence tag (FST) database for the identification of T-DNA insertion mutants in Arabidopsis thaliana JOURNAL Bioinformatics 19 (11), 1441-1442 (2003) PUBMED 12874060 REFERENCE 2 (bases 1 to 5106) AUTHORS Rosso MG, Li Y, Strizhov N, Reiss B, Dekker K, Weisshaar B. TITLE An Arabidopsis thaliana T-DNA mutagenized population (GABI-Kat) for flanking sequence tag-based reverse genetics JOURNAL Plant Mol. Biol. 53 (1-2), 247-259 (2003) PUBMED 14756321 REFERENCE 3 (bases 1 to 5106) AUTHORS Rosso MG, Li Y, Strizhov N, Weisshaar B. TITLE Direct Submission JOURNAL Submitted (20-JAN-2004) Dept. Salamini, Max-Planck-Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, Koeln D-50829, Germany REFERENCE 4 (bases 1 to 5106) TITLE Direct Submission REFERENCE 5 (bases 1 to 5106) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Bioinformatics"; date: "2003"; volume: "19"; issue: "11"; pages: "1441-1442" SGRef: number: 2; type: "Journal Article"; journalName: "Plant Mol. Biol. 53 (1-2), 247-259 (2003)" SGRef: number: 3; type: "Journal Article"; journalName: "Submitted (20-JAN-2004) Dept. Salamini, Max-Planck-Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, Koeln D-50829, Germany" SGRef: number: 4; type: "Journal Article" FEATURES Location/Qualifiers source 1..5106 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin 31..740 /label="oriV" /note="incP origin of replication" promoter 813..904 /label="AmpR promoter" CDS 905..1762 /label="AmpR" /note="beta-lactamase" rep_origin 1936..2524 /direction=RIGHT /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature 2832..2856 /label="LB T-DNA repeat" /note="left border repeat from octopine Ach5 T-DNA" regulatory 3129..3517 /label="eucaryotic promoter ubi 4-2#" /note="eucaryotic promoter ubi 4-2#" /regulatory_class="promoter" regulatory 3512..3521 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" regulatory 3518..3688 /label="pea rubisco targeting signal" /note="pea rubisco targeting signal" /regulatory_class="other" CDS 3689..4525 /gene="sulI" /label="Dihydropteroate synthase type-1" /note="Dihydropteroate synthase type-1 from Escherichia coli. Accession#: P0C002" terminator 4573..4825 /label="NOS terminator" /note="nopaline synthase terminator and poly(A) signal" misc_feature 5083..5106 /label="RB T-DNA repeat" /note="right border repeat from nopaline C58 T-DNA"
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