pADIS1 vector (V009576)

Basic Vector Information

Vector Name:
pADIS1
Antibiotic Resistance:
Ampicillin
Length:
5106 bp
Type:
Cloning vector
Replication origin:
oriV
Source/Author:
Li Y, Rosso MG, Strizhov N, Viehoever P, Weisshaar B.

pADIS1 vector Vector Map

pADIS15106 bp6001200180024003000360042004800oriVAmpR promoterAmpRoriLB T-DNA repeateucaryotic promoter ubi 4-2#pea rubisco targeting signalDihydropteroate synthase type-1NOS terminatorRB T-DNA repeat

pADIS1 vector Sequence

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Download GeneBank File(.gb)

LOCUS       V009576                 5106 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009576
VERSION     V009576
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Li Y, Rosso MG, Strizhov N, Viehoever P, Weisshaar B.
  TITLE     GABI-Kat SimpleSearch: a flanking sequence tag (FST) database for
            the identification of T-DNA insertion mutants in Arabidopsis
            thaliana
  JOURNAL   Bioinformatics 19 (11), 1441-1442 (2003)
   PUBMED   12874060
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Rosso MG, Li Y, Strizhov N, Reiss B, Dekker K, Weisshaar B.
  TITLE     An Arabidopsis thaliana T-DNA mutagenized population (GABI-Kat) for
            flanking sequence tag-based reverse genetics
  JOURNAL   Plant Mol. Biol. 53 (1-2), 247-259 (2003)
   PUBMED   14756321
REFERENCE   3  (bases 1 to 5106)
  AUTHORS   Rosso MG, Li Y, Strizhov N, Weisshaar B.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2004) Dept. Salamini, Max-Planck-Institute for
            Plant Breeding Research, Carl-von-Linne-Weg 10, Koeln D-50829,
            Germany
REFERENCE   4  (bases 1 to 5106)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5106)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Bioinformatics"; date: "2003"; volume: "19"; issue: "11"; pages:
            "1441-1442"
            SGRef: number: 2; type: "Journal Article"; journalName: "Plant Mol.
            Biol. 53 (1-2), 247-259 (2003)"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (20-JAN-2004) Dept. Salamini, Max-Planck-Institute for Plant
            Breeding Research, Carl-von-Linne-Weg 10, Koeln D-50829, Germany"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      31..740
                     /label="oriV"
                     /note="incP origin of replication"
     promoter        813..904
                     /label="AmpR promoter"
     CDS             905..1762
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1936..2524
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    2832..2856
                     /label="LB T-DNA repeat"
                     /note="left border repeat from octopine Ach5 T-DNA"
     regulatory      3129..3517
                     /label="eucaryotic promoter ubi 4-2#"
                     /note="eucaryotic promoter ubi 4-2#"
                     /regulatory_class="promoter"
     regulatory      3512..3521
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3518..3688
                     /label="pea rubisco targeting signal"
                     /note="pea rubisco targeting signal"
                     /regulatory_class="other"
     CDS             3689..4525
                     /gene="sulI"
                     /label="Dihydropteroate synthase type-1"
                     /note="Dihydropteroate synthase type-1 from Escherichia
                     coli. Accession#: P0C002"
     terminator      4573..4825
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5083..5106
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"

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