pcDNA3-hFIX vector (V008597)

LOCUS       V008597                 8223 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008597
VERSION     V008597
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8223)
  AUTHORS   Hosseini SJ, Zomorodipour A, Jalal R, Sabouni F.
  TITLE     A recombinant plasmid for specific expression of human factor IX in
            kertinocytes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8223)
  AUTHORS   Hosseini SJ, Zomorodipour A, Jalal R, Sabouni F.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2006) Molecular Genetics, The National Institute
            for Genetic Engineering and Biotechnology, Blvd. Pjoohesh. km-17
            Tehran-karaj Highway, Tehran 14155-6343, Iran
REFERENCE   3  (bases 1 to 8223)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8223)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-AUG-2006) Molecular Genetics, The National Institute for Genetic
            Engineering and Biotechnology, Blvd. Pjoohesh. km-17 Tehran-karaj
            Highway, Tehran 14155-6343, Iran"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(260..277)
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             2285..3667
                     /gene="F9"
                     /label="Coagulation factor IX"
                     /note="Coagulation factor IX from Homo sapiens. Accession#:
                     P00740"
     promoter        complement(3775..3793)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    3819..4043
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4089..4517
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4531..4861
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4928..5719
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5896..6029
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6066..6082)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6090..6106)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6114..6144)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6159..6180)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6468..7056)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7230..8087)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8088..8192)
                     /label="AmpR promoter"
     promoter        8223
                     /label="K14"
                     /note="Human keratin 14 promoter"