pcDNA3-ApoE3 vector (V008604)

LOCUS       V008604                 6516 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008604
VERSION     V008604
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Li Z, Li Y, Bi Y, Zhang H, Yao Y, Li Q, Cun W, Dong S.
  TITLE     Extracellular Interactions between Hepatitis C Virus and Secreted
            Apolipoprotein E
  JOURNAL   J. Virol. (2017) In press
REFERENCE   2  (bases 1 to 6516)
  AUTHORS   Li Z, Li Y, Bi Y, Zhang H, Yao Y, Li Q, Cun W, Dong S.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2017) The Third Department of Biological Products,
REFERENCE   3  (bases 1 to 6516)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6516)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "J. Virol.
            (2017) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-APR-2017) The Third Department of Biological Products, Institute
            of Medical Biology, Chinese Academy of Medical Sciences "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             958..1908
                     /gene="APOE"
                     /label="Apolipoprotein E"
                     /note="Apolipoprotein E from Homo sapiens. Accession#:
                     P02649"
     polyA_signal    2116..2340
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2386..2814
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2828..3157
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3224..4015
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4192..4325
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4362..4378)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4386..4402)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4410..4440)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4455..4476)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4764..5349)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5523..6380)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6381..6485)
                     /label="AmpR promoter"