pcDNA-PANX(HCVp) vector (V008614)

LOCUS       V008614                 6615 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008614
VERSION     V008614
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Zhang W, Lohman AW, Zhuravlova Y, Lu X, Wiens MD, Hoi H, Yaganoglu
            S, Mohr MA, Kitova EN, Klassen JS, Pantazis P, Thompson RJ, Campbell
            RE.
  TITLE     Optogenetic control with a photocleavable protein, PhoCl
  JOURNAL   Nat. Methods (2017) In press
   PUBMED   28288123
REFERENCE   2  (bases 1 to 6615)
  AUTHORS   Zhang W.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2017) Chemistry, University of Alberta, 11227
            Saskatchewan Drive, Department of Chemistry, CCIS 4-140, Edmonton,
            Alberta T6G2C5, Canada
REFERENCE   3  (bases 1 to 6615)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6615)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: GeneStudio v. 2.2.0.0
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat.
            Methods (2017) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-FEB-2017) Chemistry, University of Alberta, 11227 Saskatchewan
            Drive, Department of Chemistry, CCIS 4-140, Edmonton, Alberta
            T6G2C5, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             904..2181
                     /gene="PANX1"
                     /label="Pannexin-1"
                     /note="Pannexin-1 from Homo sapiens. Accession#: Q96RD7"
     polyA_signal    2215..2439
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2485..2913
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2927..3256
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3323..4114
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4291..4424
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4461..4477)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4485..4501)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4509..4539)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4554..4575)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4863..5448)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5622..6479)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6480..6584)
                     /label="AmpR promoter"