pcDNA-mBeta-cat-Tcf3 vector (V008619)

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LOCUS       40924_9851        7657 bp DNA     circular SYN 17-DEC-2018
DEFINITION  UNVERIFIED: Cloning vector pcDNA-mBeta-cat-Tcf3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7657)
  AUTHORS   Relaix F, Murphy P, Avner P, Duffy DJ.
  TITLE     MYCN Integrative Omics Reveal Context Dependent Transcriptional 
            Repressor/Activator Functions and Discover Potential Therapeutic 
            Targets
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7657)
  AUTHORS   Relaix F, Murphy P, Avner P, Duffy DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUL-2014) Systems Biology Ireland, University College 
            Dublin, Belfield, Dublin, Leinster 0004, Ireland
REFERENCE   3  (bases 1 to 7657)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7657)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-JUL-2014) Systems Biology Ireland, University College Dublin, 
            Belfield, Dublin, Leinster 0004, Ireland"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
            GenBank staff is unable to verify sequence and/or annotation 
            provided by the submitter.
FEATURES             Location/Qualifiers
     source          1..7657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(827..1206)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene;
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     promoter        complement(1391..1495)
                     /label=AmpR promoter
     enhancer        1761..2140
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2141..2344
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        2389..2407
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2440..2463
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     promoter        complement(4707..4725)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(4736..4754)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    4780..5004
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5050..5478
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5492..5821
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     polyA_signal    6855..6988
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7025..7041)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7049..7065)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7073..7103)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7118..7139)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(join(7427..7657,1..358))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"