pCDH-EF1a-GaussiaSP-MCS-IRES-zeo vector (V008631)

LOCUS       V008631                 7504 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008631
VERSION     V008631
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7504)
  AUTHORS   Kulemzin SV, Gorchakov AA, Chikaev NA, Volkova OY, Kuznetsova VV,
            Taranin AV.
  TITLE     Modular lentiviral vector system for optimization of chimeric
            antigen receptor design
  JOURNAL   Russ. J. Bioorganic Chem. (2016) In press
REFERENCE   2  (bases 1 to 7504)
  AUTHORS   Kulemzin SV, Gorchakov AA, Chikaev NA, Volkova OY, Kuznetsova VV,
            Taranin AV.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2016) Immunogenetics, Institute of Molecular and
            Cellular Biology, Lavrentyeva 8/2, Novosibirsk 630090, Russia
REFERENCE   3  (bases 1 to 7504)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7504)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Russ. J.
            Bioorganic Chem. (2016) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-AUG-2016) Immunogenetics, Institute of Molecular and Cellular
            Biology, Lavrentyeva 8/2, Novosibirsk 630090, Russia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1078..1311
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1496..1540
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1689..1730
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1807..1923
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        1966..2177
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     LTR             2190..2458
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      2484..2493
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2490..2540
                     /label="luciferase signal peptide from Gaussia princeps"
                     /note="luciferase signal peptide from Gaussia princeps"
     misc_feature    2597..3183
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             3202..3573
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     misc_feature    3583..4171
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             4245..4478
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4550..4684
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      4711..4846
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     primer_bind     complement(4879..4895)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4903..4919)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4927..4957)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4972..4993)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5281..5869)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6043..6900)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6901..7005)
                     /label="AmpR promoter"
     primer_bind     7479..7495
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"