pCD-A7M8K-p20mut vector (V008659)

LOCUS       40924_9281        8276 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCD-A7M8K-p20mut, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8276)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8276)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8276)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8276)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      927..936
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             933..2256
                     /codon_start=1
                     /note="unnamed protein product; hAPI2 fragment"
                     /protein_id="SJL86949.1"
                     /translation="MDIVENSIFLSNLMKSANTFELKYDLSCELYRMSTYSTFPAGVPV
                     SERSLARAGFYYTGVNDKVKCFCCGLMLDNWKRGDSPTEKHKKLYPSCRFVQSLNSVNN
                     LEATSQPTFPSSVTNSTHSLLPGTENSGYFRGSYSNSPSNPVNSRANQDFSALMRSSYH
                     CAMNNENARLLTFQTWPLTFLSPTDLAKAGFYYIGPGDRVACFACGGKLSNWEPKDNAM
                     SEHLRHFPKCPFIENQLQDTSRYTVSNLSMQTHAARFKTFFNWPSSVLVNPEQLASAGF
                     YYVGNSDDVKCFCCDGGLRCWESGDDPWVQHAKWFPRCEYLIRIKGQEFIRQVQASYPH
                     LLEQLLSTSDSPGDENAESSIIHFEPGEDHSEDAIMMNTPVINAAVEMGFSRSLVKQTV
                     QRKILATGENYRLVNDLVLDLLNAEDEIREEERERATEEKES"
     misc_feature    936..938
                     /label=Kozak
                     /note="Kozak"
     misc_feature    2184..2186
                     /label=PCR artefact
                     /note="PCR artefact"
     CDS             2257..3770
                     /codon_start=1
                     /note="unnamed protein product; hMLT mutated fragment"
                     /protein_id="SJL86950.1"
                     /translation="MN*IILVILIIKSKQLTSLWRRTRLPF**EI*ITGSTPSSKLLWW
                     MCTN*LTY*DSWTSKWFHCWILLNMRCVMLWMSFYSF*TREYMGYYIMQDMVMKILGTA
                     SWSPLMLQIHIGLKIVCVYKIY*N*CKKKKLDLMCSYWIWRAKEMTTMIPFQSWMH*KS
                     PPILCLDMPRVKELKLLKSSILDWQMESL*NF*KTDY*KIRKSLCYWMKLQKIWVSVTL
                     PKANRL*RFEVVYLRREHLLIQYREQNILLNLLCGIYSGPRLMNFQKVCVLSLTVVFRF
                     N*DLQLSFPMS*SSIQV*FTNHRR**CVMPTLLIFHLI*ILIQKMQIKARLKKLAATWY
                     QRIFPSIASIPDSVHCKN*RNI*SSQYVYHISTQDWKIL*RTSRK*MLGNLSLLN*TCI
                     EVWEGRLAFKLVLCLMVLTRVLQPPQEEQGIITHCKTHSMVFTIHILVIQVMLHQQIAV
                     IAAGLQMHLFQVSLTMLHVILVEVMCQ*RQLMKYHLVSLTGSEFLK"
     misc_feature    2688..2693
                     /label=C464A
                     /note="C464A"
     misc_feature    2784..2786
                     /label=stability creator
                     /note="stability creator"
     misc_feature    3246..3248
                     /label=PCR artefact
                     /note="PCR artefact"
     CDS             3780..3809
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             3825..3842
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    3871..4095
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4141..4569
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4583..4912
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4979..5770
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5949..6082
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6119..6135)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6143..6159)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6167..6197)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6212..6233)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6521..7109)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7283..8140)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8141..8245)
                     /label=AmpR promoter