Basic Vector Information
- Vector Name:
- pCC2FOS
- Antibiotic Resistance:
- Chloramphenicol
- Length:
- 8181 bp
- Type:
- Cloning vector
- Replication origin:
- ori2
- Source/Author:
- EPICENTRE Biotechnologies.
pCC2FOS vector Map
pCC2FOS vector Sequence
LOCUS 40924_9251 8181 bp DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector pCC2FOS, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8181) AUTHORS EPICENTRE Biotechnologies. TITLE Direct Submission JOURNAL Submitted (23-AUG-2007) 726 Post Road, Madison, WI 53713, USA REFERENCE 2 (bases 1 to 8181) TITLE Direct Submission REFERENCE 3 (bases 1 to 8181) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted (23-AUG-2007) 726 Post Road, Madison, WI 53713, USA" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..8181 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 288..304 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 311..329 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(485..501) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(509..525) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(533..563) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(578..599) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." CDS complement(822..1478) /codon_start=1 /label=CmR /note="chloramphenicol acetyltransferase" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" promoter complement(1479..1581) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" rep_origin 2439..3053 /label=oriV /note="origin of replication for the bacterial F plasmid" rep_origin 3129..3348 /label=ori2 /note="secondary origin of replication for the bacterial F plasmid; also known as oriS" CDS 3439..4191 /codon_start=1 /label=repE /note="replication initiation protein for the bacterial F plasmid" /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM RLSYIEKKKGRQTTHIVFSFRDITSMTTG" misc_feature 4197..4447 /label=incC /note="incompatibility region of the bacterial F plasmid" CDS 4773..5945 /codon_start=1 /label=sopA /note="partitioning protein for the bacterial F plasmid" /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR" CDS 5948..6916 /codon_start=1 /label=sopB /note="partitioning protein for the bacterial F plasmid" /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP" misc_feature 6992..7465 /label=sopC /note="centromere-like partitioning region of the bacterial F plasmid" misc_feature complement(7725..8123) /label=cos /note="lambda cos site; allows packaging into phage lambda particles" protein_bind complement(8141..8174) /label=loxP /note="Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (Shaw et al., 2021)."
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