pCBcth4 vector (V008689)

LOCUS       V008689                 5905 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008689
VERSION     V008689
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Zheng T, Cui J, Bae HR, Lynd LR, Olson DG.
  TITLE     Expression of adhA from different organisms in Clostridium
            thermocellum
  JOURNAL   Biotechnol Biofuels 10, 251 (2017)
   PUBMED   29213311
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   Zheng T.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2017) Biology, Dartmouth College, 14 Engineering
            Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 5905)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5905)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol
            Biofuels 10, 251 (2017)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-SEP-2017) Biology, Dartmouth College, 14 Engineering Drive,
            Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..209
                     /label="2638"
                     /note="2638"
                     /regulatory_class="promoter"
     CDS             210..1430
                     /codon_start=1
                     /gene="adhA"
                     /product="AdhA"
                     /label="adhA"
                     /note="derived from Thermococcus sp. strain AN1"
                     /protein_id="AUE21777.1"
                     /translation="MLWESQIPINQIFELRCRTIDYFGVGAINKFYDIAKDLKENRGIS
                     RVILVTGKSSYKKCGAWDVVKPALEENGIEYVHYDKVGPNPTVDMVDEAAQMGKEFGAQ
                     AVIGIGGGSPIDTAKSVAILLEYTDKTARELYKLQFSPRKAKPIIAINTTHGTGTEVNR
                     FAVASILEEEYKPAIAYDCIYPLYSIDDPTLMTKLPADQTRYVTIDALNHVNEAATTKV
                     ASPYSILLAKETARLIFDYLPEALIHPDNLQAKYYLLYASAIAGISFDNGLLHFTHALE
                     HPLSAVKPDLPHGLGLAILLPAVIKHIYPATARILAEIYRPLVPEVKGVPGEAELVAKK
                     VEEWLFAIGITQKLTDVGFSEDDVKRLTELAMTTPSLNLLLSLAPVEATKETIEAIYRD
                     SLYPLSK"
     gene            210..1430
                     /gene="adhA"
                     /label="adhA"
     CDS             1560..2207
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(2291..3148)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3149..3240)
                     /label="AmpR promoter"
     rep_origin      3840..4385
                     /direction=RIGHT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             4856..5857
                     /label="repB"
                     /note="RepB replication protein"