pCAT-Mgen-recA vector (V008710)

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LOCUS       40924_9076        7141 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCAT-Mgen-recA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Burgos R, Wood GE, Young L, Glass JI, Totten PA.
  TITLE     RecA mediates MgpB and MgpC phase and antigenic variation in 
            Mycoplasma genitalium, but plays a minor role in DNA repair
  JOURNAL   Mol. Microbiol. 85 (4), 669-683 (2012)
  PUBMED    22686427
REFERENCE   2  (bases 1 to 7141)
  AUTHORS   Burgos R, Wood GE, Young L, Glass JI, Totten PA.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 7141)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7141)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. 
            Microbiol."; date: "2012"; volume: "85"; issue: "4"; pages: 
            "669-683"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-MAY-2012) Synthetic Biology "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          3637..4737
                     /mol_type="other DNA"
                     /label=includes MG_338 5' end
                     /note="includes MG_338 5' end"
                     /db_xref="taxon:2097"
                     /organism="Mycoplasma genitalium"
     source          5761..6805
                     /mol_type="other DNA"
                     /label=includes MG_340 3' end
                     /note="includes MG_340 3' end"
                     /db_xref="taxon:2097"
                     /organism="Mycoplasma genitalium"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(944..1801)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(1822..2613)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      complement(2957..3385)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3526..3542
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3549..3567
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(4786..5442)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     misc_feature    6811..6871
                     /label=derived from Invitrogen TA Cloning Vector pCR-2.1
                     /note="derived from Invitrogen TA Cloning Vector pCR-2.1"
     promoter        complement(6885..6903)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6921..6937)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6945..6961)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6969..6999)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7014..7035)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."