pCamE vector (V008730)

LOCUS       40924_8941       10131 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCamE, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10131)
  AUTHORS   Ma Z, Wu L, Wang X, Li X, Li Y, Wang Z.
  TITLE     Constructing a novel plant expression vector (pCamE) with CaMV 35S 
            promoter and fifteen restriction endonuclease sites in the 
            expression cassette
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10131)
  AUTHORS   Ma Z, Wu L, Wang X, Li X, Li Y, Wang Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2012) Crop Genetics and Breeding Department, 
            Agricultural University of Hebei, South of Jianshe Street 215, 
            Baoding, Hebei 071000, P.R. China
REFERENCE   3  (bases 1 to 10131)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10131)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-SEP-2012) Crop Genetics and Breeding Department, Agricultural 
            University of Hebei, South of Jianshe Street 215, Baoding, Hebei 
            071000, P.R. China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: DNASTAR v. 7.10
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(9..25)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(33..49)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(57..87)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(102..123)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        314..992
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     CDS             1058..2080
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     polyA_signal    2124..2298
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(2376..2400)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2825..3616
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3706..4294
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(4480..4620)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4964..5158)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5227..6291)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6728..7354)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     misc_feature    complement(8656..8680)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     8883..8899
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9429..9774
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    9798..9830
                     /label=multiple cloning sites
                     /note="multiple cloning sites"
     regulatory      9851..10115
                     /label=NOS
                     /note="NOS"
                     /regulatory_class="terminator"
     terminator      9861..10113
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"