pCAMBIA1300-VE3-2S vector (V008733)

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LOCUS       V008733                11369 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008733
VERSION     V008733
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11369)
  AUTHORS   Shi XG, Zhuang WJ.
  TITLE     Binary vector pCAMBIA1300-VE3-2S for making MPBQ/MSBQ
            methyltransferase over expression in seed
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11369)
  AUTHORS   Zhuang WJ, Shi XG.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2008) Crop Science Department, Oil Crop
            Institution, Fujian Agriculture, Jinshan, Fuzhou, Fujian 350002,
            P.R. China
REFERENCE   3  (bases 1 to 11369)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11369)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-OCT-2008) Crop Science Department, Oil Crop Institution, Fujian
            Agriculture, Jinshan, Fuzhou, Fujian 350002, P.R. China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(8..260)
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(280..1293)
                     /gene="VTE3"
                     /label="2-methyl-6-phytyl-1,4-hydroquinone
                     methyltransferase, chloroplastic"
                     /note="2-methyl-6-phytyl-1,4-hydroquinone
                     methyltransferase, chloroplastic from Arabidopsis thaliana.
                     Accession#: Q9LY74"
     regulatory      1305..2449
                     /label="Brassica napus 2S seed storage protein promoter"
                     /note="Brassica napus 2S seed storage protein promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(2471..2487)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    2690..2714
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4014..4640
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             5077..6141
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      6210..6404
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6748..6888
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7074..7662)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7752..8543)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    8968..8992
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(9070..9244)
                     /label="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9287..10309)
                     /label="HygR"
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(10377..11054)
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    11245..11266
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        11281..11311
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    11319..11335
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11343..11359
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"