pCAGGShMnSOD vector (V008789)

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LOCUS       V008789                 6345 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008789
VERSION     V008789
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6345)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6345)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6345)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    6..39
                     /label="5UTR"
                     /note="5UTR"
     CDS             40..705
                     /gene="SOD2"
                     /label="Superoxide dismutase [Mn], mitochondrial"
                     /note="Superoxide dismutase [Mn], mitochondrial from Homo
                     sapiens. Accession#: P04179"
     misc_feature    709..801
                     /label="3UTR"
                     /note="3UTR"
     polyA_signal    954..1009
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     polyA_signal    1359..1493
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     regulatory      complement(1359..1433)
                     /label="polyA"
                     /note="polyA"
                     /regulatory_class="terminator"
     regulatory      1359..1433
                     /label="polyA"
                     /note="polyA"
                     /regulatory_class="terminator"
     misc_feature    1489..2234
                     /label="PY"
                     /note="PY"
     rep_origin      2122..2234
                     /label="PY-O"
                     /note="PY-O"
     rep_origin      2336..2471
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     polyA_signal    2491..2625
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2863..3451)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3625..4482)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4483..4587)
                     /label="AmpR promoter"
     enhancer        4618..4997
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    4618..4995
                     /label="hCMV"
                     /note="hCMV"
     promoter        4999..5276
                     /label="chicken beta-actin promoter"
     intron          5277..6294
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"