Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V012214 | pLSLZ.D.R | In stock, 1 week for quality controls |
Buy one, get one free! |
Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.
Basic Vector Information
- Vector Name:
- pLSLZ.D.R
- Antibiotic Resistance:
- Kanamycin
- Length:
- 15212 bp
- Type:
- Plant Expression Vectors
- Replication origin:
- ori
- Host:
- Plants
- Promoter:
- CaMV 35S (enhanced)
pLSLZ.D.R vector Map
Plasmid Protocol
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5. Store the plasmid at -20 ℃.
6. The concentration of plasmid re-measurement sometimes differs from the nominal value, which may be due to the position of the lyophilized plasmid in the tube, the efficiency of the re-dissolution, the measurement bias, and adsorption on the wall of the tube, therefore, it is recommended to transform and extract the plasmid before using it
General Plasmid Transform Protocol
1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do not stir, and then ice bath for 2min.
2. Add 900μl of LB liquid medium without antibiotics, and incubate at 37℃ for 45min (30℃ for 1-1.5 hours) with 180rpm shaking.
3. Centrifuge at 6000rpm for 5min, leave only 100μl of supernatant to resuspend the bacterial precipitate and spread it onto the target plasmid-resistant LB plate.
4. Invert the plate and incubate at 37℃ for 14h, or at 30℃ for 20h.
5. Pick a single colony into LB liquid medium, add the corresponding antibiotics, incubate at 220rpm for 14h, and extract the plasmid according to the experimental needs and the instructions of the plasmid extraction kit.
pLSLZ.D.R vector Sequence
LOCUS V012214 15212 bp DNA circular SYN 13-JAN-2022
DEFINITION Exported.
ACCESSION V012214
VERSION V012214
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 15212)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 15212)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..15212
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 1129..1323
/label="pVS1 oriV"
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 1667..1807
/label="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(1993..2581)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2671..3462)
/label="KanR"
/note="aminoglycoside phosphotransferase"
misc_feature 3887..3911
/label="LB T-DNA repeat"
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal complement(3989..4163)
/label="CaMV poly(A) signal"
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(4204..5214)
/label="HygR"
/note="aminoglycoside phosphotransferase from E. coli"
promoter complement(5253..5930)
/label="CaMV 35S promoter (enhanced)"
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
promoter 6506..7177
/label="CaMV 35S promoter (enhanced)"
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
protein_bind 7237..7261
/label="attB1"
/note="recombination site for the Gateway(R) BP reaction"
CDS 7295..7315
/label="SV40 NLS"
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
CDS 7349..7525
/gene="EGR1"
/label="Early growth response protein 1"
/note="Early growth response protein 1 from Serinus
canaria. Accession#: O73694"
CDS 7583..8170
/label="FokI cleavage domain"
/note="nonspecific DNA cleavage domain of the FokI
endonuclease (Li et al., 1992)"
terminator 8184..8436
/label="NOS terminator"
/note="nopaline synthase terminator and poly(A) signal"
protein_bind 8481..8501
/label="attB5"
/note="core recombination site for the Gateway(R) BP
reaction"
CDS 10008..10796
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase"
terminator 10826..11078
/label="NOS terminator"
/note="nopaline synthase terminator and poly(A) signal"
promoter complement(11103..11121)
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS complement(11572..12447)
/note="Replication-associated protein A from Bean yellow
dwarf virus. Accession#: O39521"
/label="Replication-associated protein A"
misc_feature 12821..12845
/label="RB T-DNA repeat"
/note="right border repeat from nopaline C58 T-DNA"
CDS 14145..14771
/label="pVS1 StaA"
/note="stability protein from the Pseudomonas plasmid pVS1
(Heeb et al., 2000)"
CDS join(15208..15212,1..1060)
/label="pVS1 RepA"
/note="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"