pC1300intA vector (V008965)

LOCUS       40924_7891        9148 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pC1300intA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9148)
  AUTHORS   Ouwerkerk PBF.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-2000) Dept. of Molecular Cell Biology, Institute 
            for Molecular Plant Sciences, Wassenaarseweg 64, Leiden 2333 AL, 
            Netherlands
REFERENCE   2  (bases 1 to 9148)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9148)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (11-AUG-2000) Dept. of Molecular Cell Biology, Institute for 
            Molecular Plant Sciences, Wassenaarseweg 64, Leiden 2333 AL, 
            Netherlands"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..57
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(61..77)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    280..304
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1604..2230
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             2667..3731
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      3800..3994
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    4338..4478
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4664..5252)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5342..6133)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    6558..6582
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(6660..6834)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     exon            complement(6874..7566)
                     /gene="hptII"
                     /number=2
     intron          complement(7561..7750)
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     exon            complement(7757..8089)
                     /gene="hptII"
                     /number=1
     promoter        complement(8157..8834)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    9025..9046
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9061..9091
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9099..9115
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9123..9139
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"