pC-N vector (V008969)

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LOCUS       V008969                 6225 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008969
VERSION     V008969
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Bergeron E, Zivcec M, Chakrabarti AK, Nichol ST, Albarino CG,
            Spiropoulou CF.
  TITLE     Recovery of Recombinant Crimean Congo Hemorrhagic Fever Virus
            Reveals a Function for Non-structural Glycoproteins Cleavage by
            Furin
  JOURNAL   PLoS Pathog. 11 (5), E1004879 (2015)
   PUBMED   25933376
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   Bergeron E, Chakrabarti AK, Nichol ST, Albarino CG, Spiropoulou CF.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2014) Viral Special Pathogens Branch, Centers for
            Disease Control and Prevention, 1600 Clifton Rd NE, Atlanta, GA
            30333, USA
REFERENCE   3  (bases 1 to 6225)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6225)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS
            Pathog."; date: "2015"; volume: "11"; issue: "5"; pages: "E1004879"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-MAR-2014) Viral Special Pathogens Branch, Centers for Disease
            Control and Prevention, 1600 Clifton Rd NE, Atlanta, GA 30333, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      140..384
                     /label="CMV IE"
                     /note="CMV IE"
                     /regulatory_class="enhancer"
     regulatory      366..663
                     /label="chicken beta actin promoter"
                     /note="chicken beta actin promoter"
                     /regulatory_class="promoter"
     promoter        386..661
                     /label="chicken beta-actin promoter"
     intron          662..1670
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             1751..3196
                     /gene="N"
                     /label="Nucleoprotein"
                     /note="Nucleoprotein from Crimean-Congo hemorrhagic fever
                     virus (strain Nigeria/IbAr10200/1970). Accession#: P89522"
     polyA_signal    3314..3369
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(3728..3744)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3752..3768)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3776..3806)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3821..3842)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3900..4096
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    4102..4236
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4475..5063)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5237..6094)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6095..6199)
                     /label="AmpR promoter"