Basic Vector Information
- Vector Name:
- pBSTG1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3795 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Tiede C, Tang AA, Deacon SE, Mandal U, Nettleship JE, Owen RL, George SE, Harrison DJ, Owens RJ, Tomlinson DC, McPherson MJ.
pBSTG1 vector Vector Map
pBSTG1 vector Sequence
LOCUS 40924_7466 3795 bp DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector pBSTG1, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3795) AUTHORS Tiede C, Tang AA, Deacon SE, Mandal U, Nettleship JE, Owen RL, George SE, Harrison DJ, Owens RJ, Tomlinson DC, McPherson MJ. TITLE Adhiron: a stable and versatile peptide display scaffold for molecular recognition applications JOURNAL Protein Eng. Des. Sel. 27 (5), 145-155 (2014) PUBMED 24668773 REFERENCE 2 (bases 1 to 3795) AUTHORS Deacon SE, Tiede C, Tomlinson DC, McPherson MJ. TITLE Direct Submission JOURNAL Submitted (19-FEB-2014) School of Molecular and Cellular Biology, University of Leeds, Astbury Building, Leeds, West Yorkshire WF12 0LN, UK REFERENCE 3 (bases 1 to 3795) TITLE Direct Submission REFERENCE 4 (bases 1 to 3795) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Protein Eng. Des. Sel."; date: "2014"; volume: "27"; issue: "5"; pages: "145-155" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (19-FEB-2014) School of Molecular and Cellular Biology, University of Leeds, Astbury Building, Leeds, West Yorkshire WF12 0LN, UK" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..3795 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 1..31 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 39..55 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 63..79 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" misc_feature 131..187 /label=DsbA secretion signal sequence /note="DsbA secretion signal sequence" CDS 236..253 /label=6xHis /note="6xHis affinity tag" misc_feature 257..301 /note="flexible linker between oligohistidine tag and truncated M13 gp3 coding region" CDS 302..772 /codon_start=1 /product="truncated gp3" /label=truncated gp3 /note="minor coat protein" /protein_id="AIE57570.1" /translation="GGSGSGDFDYEKMANANKGAMTENADENALQSDAKGKLDSVATDY GAAIDGFIGDVSGLANGNGATGDFAGSNSQMAQVGDGDNSPLMNNFRQYLPSLPQSVEC RPYVFGAGKPYEFSIDCDKINLFRGVFAFLLYVATFMYVFSTFANILRNKES" primer_bind complement(782..798) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 1011..1466 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 1748..1852 /label=AmpR promoter CDS 1853..2710 /label=AmpR /note="beta-lactamase" rep_origin 2884..3472 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 3760..3781 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP."
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