pBSk-BV4 vector (V009032)

LOCUS       40924_7431        3378 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBSk-BV4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Chakrabarty PK, Kalbande BB, Chavhan RL, Warade JG, Narwade AV, 
            Sable SV, Monga D, Mayee CD.
  TITLE     Inverted repeat constructs for RNA interference-mediated targeting 
            of movement/pre-coat protein genes of Cotton leaf curl virus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Chakrabarty PK, Kalbande BB, Chavhan RL, Warade JG, Narwade AV, 
            Sable SV, Monga D, Mayee CD.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2010) Division of Crop Improvement, Central 
            Institute for Cotton Research, Post Bag No. 02, Shankar Nagar P.O., 
            Nagpur, Maharashtra 440010, India
REFERENCE   3  (bases 1 to 3378)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3378)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-DEC-2010) Division of Crop Improvement, Central Institute for 
            Cotton Research, Post Bag No. 02, Shankar Nagar P.O., Nagpur, 
            Maharashtra 440010, India"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3378
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    653..1177
                     /note="MCS; multiple cloning site"
     misc_feature    659..835
                     /note="Cotton leaf curl virus isolate Sirsa (Haryana)
                     beta-V4 protein fragment"
     intron          842..964
                     /note="chitinase intron 1; derived from Gossypium hirsutum"
     misc_feature    complement(971..1147)
                     /note="Cotton leaf curl virus isolate Sirsa (Haryana)
                     beta-V4 protein fragment"
     promoter        complement(1190..1208)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1229..1245)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1253..1269)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1277..1307)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1322..1343)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1631..2219)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2393..3250)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3251..3355)
                     /label=AmpR promoter