pBSc241C vector (V009057)

LOCUS       V009057                 4520 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009057
VERSION     V009057
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Radeck J, Meyer D, Lautenschlager N, Mascher T.
  TITLE     Bacillus SEVA siblings: A Golden Gate-based toolbox to create
            personalized integrative vectors for Bacillus subtilis
  JOURNAL   Sci Rep 7 (1), 14134 (2017)
   PUBMED   29074996
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Radeck J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2017) Biology, Technische Universitat Dresden,
            Zellescher Weg 20b, Dresden 01627, Germany
REFERENCE   3  (bases 1 to 4520)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4520)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep";
            date: "2017"; volume: "7"; issue: "1"; pages: "14134"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-APR-2017) Biology, Technische Universitat Dresden, Zellescher
            Weg 20b, Dresden 01627, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4520
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    56..112
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     terminator      155..249
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             428..1240
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     oriT            1410..1518
                     /label="oriT"
                     /note="incP origin of transfer"
     rep_origin      1612..2200
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2257..3087)
                     /label="pRO1600 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     rep_origin      3101..3452
                     /label="pRO1600 oriV"
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             complement(3620..4267)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     terminator      complement(4428..4514)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"