pBrachyTAG-G217 vector (V009101)

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LOCUS       V009101                 6855 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009101
VERSION     V009101
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6855)
  AUTHORS   Thole V, Worland B, Schieszl K, Wright JM, Vain P.
  TITLE     pBrachyTAG vector system: New tools for DNA tagging and trapping in
            grasses
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6855)
  AUTHORS   Thole V, Vain P.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAR-2010) Crop Genetics, John Innes Centre, Colney
            Lane, Norwich NR4 7UH, UK
REFERENCE   3  (bases 1 to 6855)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6855)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-MAR-2010) Crop Genetics, John Innes Centre, Colney Lane, Norwich
            NR4 7UH, UK"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(61..649)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(823..1635)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1926..2361
                     /label="pSa ori"
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    2508..2532
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    2533..2558
                     /note="26-nt long spacer sequence separating left border
                     No.1 and No.2 of the double left border sequence (Thole et
                     al., 2007)"
     misc_feature    2559..2583
                     /note="nopaline-type T-DNA left border sequence No.2
                     belonging to a double left border sequence separated by a
                     26-nt long spacer sequence from left border No.1 (Thole et
                     al., 2007)"
     misc_feature    2598..2693
                     /note="UniBox1; synthetic 96-nt long unique sequence
                     containing forward and reverse primer sequences;
                     '>http://www.brachytag.org/vector-system.htm"
     terminator      complement(2713..2965)
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     intron          complement(3693..3882)
                     /label="cat1 intron"
                     /note="castor bean catalase intron, modified"
     promoter        complement(4231..4575)
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      complement(4678..4930)
                     /note="NOS-ter; poly(A) signal of nopaline synthase gene"
                     /regulatory_class="terminator"
     terminator      4678..4930
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4964..5680)
                     /label="EGFP"
                     /note="enhanced GFP"
     misc_feature    5700..5724
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    5725..5748
                     /label="right border overdrive sequence"
                     /note="right border overdrive sequence"
     CDS             5952..6752
                     /gene="virG"
                     /label="Regulatory protein VirG"
                     /note="Regulatory protein VirG from Agrobacterium
                     tumefaciens (strain 15955). Accession#: P62722"