pBPHLWL vector (V009108)

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LOCUS       40924_7001        7963 bp DNA     circular SYN 17-DEC-2018
DEFINITION  InSITE injectable donor vector pBPHLWL, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7963)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR.
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns
  JOURNAL   Nat. Methods 8 (3), 231-237 (2011)
  PUBMED    21473015
REFERENCE   2  (bases 1 to 7963)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin C-C., Potter 
            CJ, Clandinin TR.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2011) Neurobiology, Stanford University, 299 W. 
            Campus Dr., Stanford, CA 94305, USA
REFERENCE   3  (bases 1 to 7963)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7963)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Methods"; date: "2011"; volume: "8"; issue: "3"; pages: "231-237"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-JAN-2011) Neurobiology, Stanford University, 299 W. Campus Dr., 
            Stanford, CA 94305, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    689..758
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     primer_bind     949..965
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      968..1074
                     /label=P transposase promoter
                     /note="P transposase promoter"
                     /regulatory_class="promoter"
     3'UTR           1102..1362
                     /label=hsp70 3'UTR
                     /note="hsp70 3'UTR"
     misc_recomb     1383..1416
                     /label=loxP
                     /note="loxP"
     protein_bind    1383..1416
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     gene            1592..5711
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     protein_bind    complement(5717..5750)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(5775..5793)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5814..5830)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5838..5854)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5862..5892)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5907..5928)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6216..6804)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6978..7835)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7836..7940)
                     /label=AmpR promoter