pBOMB4R-MCI vector (V009111)

LOCUS       V009111                10768 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009111
VERSION     V009111
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10768)
  AUTHORS   Bauler LD, Hackstadt T.
  TITLE     Expression and Targeting of Secreted Proteins from Chlamydia
            trachomatis
  JOURNAL   J. Bacteriol. 196 (7), 1325-1334 (2014)
   PUBMED   24443531
REFERENCE   2  (bases 1 to 10768)
  AUTHORS   Bauler LD, Hackstadt T.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2013) Laboratory of Intracellular Parasites, Rocky
            Mountain Laboratories, NIAID, NIH, 903 S. Fourth St., Hamilton, MT
            59840, USA
REFERENCE   3  (bases 1 to 10768)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10768)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "2014"; volume: "196"; issue: "7"; pages:
            "1325-1334"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-OCT-2013) Laboratory of Intracellular Parasites, Rocky Mountain
            Laboratories, NIAID, NIH, 903 S. Fourth St., Hamilton, MT 59840,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(5055..6407)
                     /note="Probable plasmid replicative DNA helicase from
                     Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC
                     VR-902B). Accession#: B0BCM3"
                     /label="Probable plasmid replicative DNA helicase"
     regulatory      7555..7742
                     /gene="rpoB"
                     /regulatory_class="promoter"
     gene            7555..7742
                     /gene="rpoB"
                     /label="rpoB"
     misc_feature    7743..7777
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     promoter        7778..7849
                     /label="AmpR promoter"
     misc_feature    8034..8413
                     /label="ISS"
                     /note="immunostimulatory sequence from the AmpR gene;
                     contains unmethylated CpG dinucleotides in the context of
                     5'-AACGTT-3' (Sato et al., 1996)"
     rep_origin      8881..9468
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             10004..10711
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"