pBLOXNPT vector (V009154)

LOCUS       40924_6696       14961 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pBLOXNPT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14961)
  AUTHORS   Ma BG, Ma C, Niu JX.
  TITLE     Gene expression controlled by inducible Cre/loxP recombination 
            system in transgenic tobacco
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14961)
  AUTHORS   Ma BG, Ma C, Niu JX.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2007) Department of Horticulture, Agriculture 
            College Shihezi University, Shihezi, Xinjiang 832000, P.R. China
REFERENCE   3  (bases 1 to 14961)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14961)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-DEC-2007) Department of Horticulture, Agriculture College 
            Shihezi University, Shihezi, Xinjiang 832000, P.R. China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(148..516)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(549..658)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      complement(790..1168)
                     /direction=LEFT
                     /note="ColE1 ori; similar to sequence in GenBank Accession 
                     Number V00268"
     CDS             complement(1342..1989)
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
     misc_feature    complement(2454..2478)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2511..2529
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(2583..2616)
                     /label=loxP site
                     /note="loxP site"
     protein_bind    2583..2616
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        2776..2959
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2983..3771
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     rep_origin      complement(4338..4963)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     protein_bind    5026..5047
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5062..5092
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5100..5116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5124..5140
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5664..6009
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             6048..7856
                     /label=GUS
                     /note="beta-glucuronidase"
     regulatory      7930..8182
                     /label=NOS
                     /note="NOS"
                     /regulatory_class="terminator"
     terminator      7930..8182
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(8191..8207)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(8825..8849)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    complement(9359..10401)
                     /label=similar to tetA in GenBank Accession Number L13842
                     /note="similar to tetA in GenBank Accession Number L13842"
     CDS             complement(10448..11593)
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     CDS             complement(11895..12686)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     mobile_element  12878..13645
                     /label=IS1
                     /note="prokaryotic transposable element"
     misc_feature    complement(13647..13997)
                     /note="similarity to NPT III gene in GenBank Accession
                     Number V01547"
     misc_feature    complement(13998..14269)
                     /label=similar to kilA in GenBank Accession Number M62846
                     /note="similar to kilA in GenBank Accession Number M62846"
     rep_origin      complement(14346..14956)
                     /direction=LEFT
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"