pblDHC1m vector (V009159)

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LOCUS       V009159                 4094 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009159
VERSION     V009159
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Liu TZ.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2013) Guangzhou Institutes of Biomedicine and
            Health, Chinese Academy of Sciences, 190 Kaiyuan Road, Science Park,
            Guangdong, Guangzhou 510530, China
REFERENCE   2  (bases 1 to 4094)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4094)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (06-OCT-2013) Guangzhou Institutes of Biomedicine and Health,
            Chinese Academy of Sciences, 190 Kaiyuan Road, Science Park,
            Guangdong, Guangzhou 510530, China"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label="AmpR promoter"
     CDS             129..986
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1160..1748
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2036..2057
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2171..2189
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2226..2242
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      2272..2321
                     /label="artifical promoter"
                     /note="artifical promoter"
                     /regulatory_class="promoter"
     misc_feature    2277..2304
                     /label="MTB dif"
                     /note="MTB dif"
     CDS             2366..3361
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     misc_feature    3377..3404
                     /label="MTB dif"
                     /note="MTB dif"
     promoter        complement(3451..3469)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3479..3495)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      3637..4092
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"