pBJ2 vector (V009176)

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LOCUS       40924_6566        8005 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle expression vector pBJ2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8005)
  AUTHORS   Equbal MJ, Agarwal GP.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   2  (bases 1 to 8005)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8005)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (01-JUN-2010) Department of Biochemical Engineering "
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(26..73)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(140..157)
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    complement(158..203)
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             complement(207..239)
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             complement(243..260)
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             complement(270..287)
                     /label=6xHis
                     /note="6xHis affinity tag"
     RBS             complement(306..328)
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(343..367)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(368..386)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             461..1117
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        1672..1749
                     /label=lacI promoter
     misc_feature    2701..5451
                     /note="derived from BL1 replicon of Corynebacterium
                     glutamicum plasmid pBL1 INSD accession X03987; involved in 
                     plasmid pBJ1 replication in Corynebacterium glutamicum"
     misc_feature    5593..5735
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5921..6509)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             6631..7443
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(7539..7994)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"