pENTR4-FLAG vector (V010494)

MSDS

Basic Vector Information

Vector Name:: pENTR4-FLAG

Antibiotic Resistance:: Kanamycin

Length:: 3401 bp

Type:: Gateway vectors

Replication origin:: ori

Copy Number:: Low copy number

Cloning Method:: Enzyme digestion and ligation

5' Primer:: ENTR-F: CTACAAACTCTTCCTGTTAGTTAG

3' Primer:: ENTR-R: ATGGCTCATAACACCCCTTG

Fusion Tag:: N-FLAG

pENTR4-FLAG vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pENTR4-FLAG vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_17532        3401 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3401)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     protein_bind    358..457
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             466..489
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     protein_bind    complement(562..661)
                     /gene="mutant version of attL"
                     /label=LR Clonase(TM) binding site
                     /bound_moiety="LR Clonase(TM)"
                     /note="attL2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             784..1590
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1683..2271
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      2562..2605
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2737..2764
                     /note="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     protein_bind    2828..2927
                     /gene="mutant version of attL"
                     /label=LR Clonase(TM) binding site
                     /bound_moiety="LR Clonase(TM)"
                     /note="attL1"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2936..2959
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     protein_bind    complement(3032..3131)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"

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