pAC_SalSer914 vector (V009583)

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LOCUS       V009583                 3854 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009583
VERSION     V009583
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Anderson JC, Voigt CA, Arkin AP.
  TITLE     Environmental signal integration by a modular AND gate
  JOURNAL   Mol. Syst. Biol. 3, 133 (2007)
   PUBMED   17700541
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Salis HM, Mirsky EA, Voigt CA.
  TITLE     Automated design of synthetic ribosome binding sites to control
            protein expression
  JOURNAL   Nat. Biotechnol. 27 (10), 946-950 (2009)
   PUBMED   19801975
REFERENCE   3  (bases 1 to 3854)
  AUTHORS   Tamsir A, Tabor JJ, Voigt CA.
  TITLE     Robust multicellular computing using genetically encoded NOR gates
            and chemical 'wires'
  JOURNAL   Nature 469 (7329), 212-215 (2011)
   PUBMED   21150903
REFERENCE   4  (bases 1 to 3854)
  AUTHORS   Moser F, Voigt CA.
  TITLE     Genetic Circuit Performance under Conditions Relevant for Industrial
            Bioreactors
  JOURNAL   Unpublished
REFERENCE   5  (bases 1 to 3854)
  AUTHORS   Anderson JC, Moser F, Tamsir A, Salis H.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JAN-2012) Biological Engineering, MIT, 500 Tech
            Square, Rm 209D, Cambridge, MA 02139, USA
REFERENCE   6  (bases 1 to 3854)
  TITLE     Direct Submission
REFERENCE   7  (bases 1 to 3854)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Syst.
            Biol. 3, 133 (2007)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Nat.
            Biotechnol."; date: "2009"; volume: "27"; issue: "10"; pages:
            "946-950"
            SGRef: number: 3; type: "Journal Article"; journalName: "Nature";
            date: "2011"; volume: "469"; issue: "7329"; pages: "212-215"
            SGRef: number: 4; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 5; type: "Journal Article"; journalName: "Submitted
            (06-JAN-2012) Biological Engineering, MIT, 500 Tech Square, Rm 209D,
            Cambridge, MA 02139, USA"
            SGRef: number: 6; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(221..1120)
                     /gene="nahR"
                     /label="HTH-type transcriptional activator NahR"
                     /note="HTH-type transcriptional activator NahR from
                     Pseudomonas putida. Accession#: P10183"
     gene            1282..1371
                     /gene="supD"
                     /label="supD"
     CDS             1431..1460
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             1476..1493
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      1719..1765
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1946..2602)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2603..2705)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(3231..3776)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."