pACW-hyg vector (V009590)

Download GeneBank File(.gb)

LOCUS       V009590                 4012 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009590
VERSION     V009590
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Myronovskyi M, Broetz E, Rosenkraenzer B, Manderscheid N, Rebets Y,
            Luzhetskyy A.
  TITLE     From synthetic promoters to new antibiotics: transcriptional
            activation of silent landomycin A cluster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Myronovskyi M, Broetz E, Rosenkraenzer B, Manderscheid N, Rebets Y,
            Luzhetskyy A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2014) AMEG, Helmholtz Institute for Pharmaceutical
            Research Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes,
            Saarbruecken, Saarland 66123, Germany
REFERENCE   3  (bases 1 to 4012)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4012)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-DEC-2014) AMEG, Helmholtz Institute for Pharmaceutical Research
            Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes,
            Saarbruecken, Saarland 66123, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      434..474
                     /gene="D6"
                     /regulatory_class="promoter"
     gene            434..474
                     /gene="D6"
                     /label="D6"
     misc_feature    475..524
                     /label="P-GG"
                     /note="P-GG"
     CDS             632..1627
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     misc_feature    1637..1687
                     /label="B-CC"
                     /note="B-CC"
     regulatory      1694..1734
                     /gene="D11"
                     /regulatory_class="promoter"
     gene            1694..1734
                     /gene="D11"
                     /label="D11"
     primer_bind     complement(1791..1807)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1815..1831)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1839..1869)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1884..1905)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2193..2781)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2955..3812)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3813..3917)
                     /label="AmpR promoter"