pAcUW31 vector (V009591)

Download GeneBank File(.gb)

LOCUS       V009591                 8532 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009591
VERSION     V009591
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8532)
  AUTHORS   Kitts PA.
  TITLE     CLONTECH Vectors On Disc version 1.3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8532)
  AUTHORS   Kitts PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc.,
            1020 East Meadow Circle, Palo Alto, CA 94303, USA
REFERENCE   3  (bases 1 to 8532)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8532)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East
            Meadow Circle, Palo Alto, CA 94303, USA"
            SGRef: number: 3; type: "Journal Article"
            This vector can be obtained from CLONTECH Laboratories, Inc., 1020
            East Meadow Circle, Palo Alto, CA 94303, USA. To place an order call
            (415) 424-8222 or (800) 662-2566, extension 1. International
            customers, please contact your local distributor. For technical
            information, call (415) 424- 8222 or (800) 662-2566, extension 3.
            This sequence has been compiled from information in the sequence
            databases, published literature and other sources, together with
            partial sequences obtained by CLONTECH. If you suspect there is an
            error in this sequence, please contact CLONTECH's Technical Service
            Department at (415) 424-8222 or (800) 662-2566, extension 3 or
            E-mail TECH@CLONTECH.COM.
FEATURES             Location/Qualifiers
     source          1..8532
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             761..1390
                     /gene="LEF-2"
                     /label="Primase-associated factor LEF-2"
                     /note="Primase-associated factor LEF-2 from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     1269..2096
                     /label="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     polyA_signal    complement(2102..2236)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(2565..2674)
                     /label="p10 promoter"
                     /note="baculovirus promoter for expression in insect cells"
     promoter        2822..2913
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     misc_recomb     2925..4282
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     primer_bind     complement(5426..5442)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      5655..6110
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6392..6496
                     /label="AmpR promoter"
     CDS             6497..7354
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7528..8116
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8404..8425
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8440..8470
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8478..8494
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8502..8518
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"