pACS-hyg vector (V009601)

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LOCUS       V009601                 4020 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009601
VERSION     V009601
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Myronovskyi M, Broetz E, Rosenkraenzer B, Manderscheid N, Rebets Y,
            Luzhetskyy A.
  TITLE     From synthetic promoters to new antibiotics: transcriptional
            activation of silent landomycin A cluster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Myronovskyi M, Broetz E, Rosenkraenzer B, Manderscheid N, Rebets Y,
            Luzhetskyy A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2014) AMEG, Helmholtz Institute for Pharmaceutical
            Research Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes,
            Saarbruecken, Saarland 66123, Germany
REFERENCE   3  (bases 1 to 4020)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4020)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-DEC-2014) AMEG, Helmholtz Institute for Pharmaceutical Research
            Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes,
            Saarbruecken, Saarland 66123, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      445..488
                     /gene="A1-14"
                     /regulatory_class="promoter"
     gene            445..488
                     /gene="A1-14"
                     /label="A1-14"
     misc_feature    495..545
                     /label="B-CC"
                     /note="B-CC"
     CDS             653..1648
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     misc_feature    1658..1707
                     /label="P-GG"
                     /note="P-GG"
     regulatory      1708..1748
                     /gene="P21"
                     /regulatory_class="promoter"
     gene            1708..1748
                     /gene="P21"
                     /label="P21"
     primer_bind     complement(1799..1815)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1823..1839)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1847..1877)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1892..1913)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2201..2789)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2963..3820)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3821..3925)
                     /label="AmpR promoter"