pACM-aadA vector (V009607)

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LOCUS       40924_3821        3828 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pACM-aadA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Myronovskyi M, Broetz E, Rosenkraenzer B, Manderscheid N, Rebets Y, 
            Luzhetskyy A.
  TITLE     From synthetic promoters to new antibiotics: transcriptional 
            activation of silent landomycin A cluster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Myronovskyi M, Broetz E, Rosenkraenzer B, Manderscheid N, Rebets Y, 
            Luzhetskyy A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2014) AMEG, Helmholtz Institute for Pharmaceutical
            Research Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes, 
            Saarbruecken, Saarland 66123, Germany
REFERENCE   3  (bases 1 to 3828)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3828)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-DEC-2014) AMEG, Helmholtz Institute for Pharmaceutical Research 
            Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes, 
            Saarbruecken, Saarland 66123, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      440..480
                     /gene="B6"
                     /regulatory_class="promoter"
     gene            440..480
                     /gene="B6"
                     /label=B6
     misc_feature    481..530
                     /label=P-GG
                     /note="P-GG"
     CDS             652..1440
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     misc_feature    1453..1503
                     /label=B-CC
                     /note="B-CC"
     regulatory      1510..1550
                     /gene="B5"
                     /regulatory_class="promoter"
     gene            1510..1550
                     /gene="B5"
                     /label=B5
     primer_bind     complement(1607..1623)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1631..1647)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1655..1685)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1700..1721)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2009..2597)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2771..3628)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3629..3733)
                     /label=AmpR promoter