pAc5.1B_FN_su(ur) vector (V009610)

Download GeneBank File(.gb)

LOCUS       V009610                 8245 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009610
VERSION     V009610
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8245)
  AUTHORS   Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN,
            Belyaeva ES, Belyakin SN.
  TITLE     Repressive Histone Marks Renewal Impedes Replication Fork
            Progression and Contributes To Late Replication in Drosophila
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8245)
  AUTHORS   Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN,
            Belyaeva ES, Belyakin SN.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUN-2016) Genomics lab, IMCB SD RAS, Lavrentjeva ave.
            8/2, Novosibirsk, NSO 630090, Russia
REFERENCE   3  (bases 1 to 8245)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8245)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-JUN-2016) Genomics lab, IMCB SD RAS, Lavrentjeva ave. 8/2,
            Novosibirsk, NSO 630090, Russia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label="Ac5 promoter"
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2635..5520
                     /gene="SuUR"
                     /label="Protein suppressor of underreplication"
                     /note="Protein suppressor of underreplication from
                     Drosophila melanogaster. Accession#: Q9VTE2"
     regulatory      5528..5537
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5566..5631
                     /label="3xFLAG"
                     /note="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
     polyA_signal    complement(5671..5805)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(6427..7015)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7189..8046)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8047..8151)
                     /label="AmpR promoter"