pABC5'-FlagHis vector (V009632)

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LOCUS       V009632                 7009 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009632
VERSION     V009632
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7009)
  AUTHORS   Lamping E, Monk BC, Niimi K, Holmes AR, Tsao S, Tanabe K, Niimi M,
            Uehara Y, Cannon RD.
  TITLE     Characterization of three classes of membrane proteins involved in
            fungal azole resistance by functional hyperexpression in
            Saccharomyces cerevisiae
  JOURNAL   Eukaryotic Cell 6 (7), 1150-1165 (2007)
   PUBMED   17513564
REFERENCE   2  (bases 1 to 7009)
  AUTHORS   Lamping E, Monk BC, Niimi K, Holmes AR, Tsao S, Kaneko A, Niimi M,
            Uehara Y, Cannon RD.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2006) Oral Sciences, Univ. of Otago, 310 Gt. King
            St, Dunedin, Otago 9001, New Zealand
REFERENCE   3  (bases 1 to 7009)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7009)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryotic
            Cell"; date: "2007"; volume: "6"; issue: "7"; pages: "1150-1165"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-AUG-2006) Oral Sciences, Univ. of Otago, 310 Gt. King St,
            Dunedin, Otago 9001, New Zealand"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        623..641
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      687..1816
                     /label="Saccharomyces cerevisiae PDR5"
                     /note="Saccharomyces cerevisiae PDR5"
                     /regulatory_class="promoter"
     misc_feature    1817..1854
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     CDS             1855..1878
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             1879..1896
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      1974..2244
                     /label="PGK1 terminator"
                     /note="transcription terminator for 3-phosphoglycerate
                     kinase gene"
     CDS             3228..4118
                     /gene="HIS1"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P00498"
     misc_feature    4500..4767
                     /label="Saccharomyces cerevisiae PDR5 gene, 3' end"
                     /note="Saccharomyces cerevisiae PDR5 gene, 3' end"
     promoter        complement(4821..4839)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4860..4876)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4884..4900)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4908..4938)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4953..4974)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5262..5850)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6024..6881)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6882..6986)
                     /label="AmpR promoter"