pAB32 vector (V009655)

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LOCUS       40924_3351        9121 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAB32, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9121)
  AUTHORS   Bradley AS, Pearson A, Saenz JP, Marx CJ.
  TITLE     Adenosylhopane: the first step in biosynthesis of hopanoid side 
            chains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9121)
  AUTHORS   Bradley AS, Pearson A, Saenz JP, Marx CJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2010) OEB, Harvard University, 16 Divinity Ave., 
            Cambridge, MA 02138, USA
REFERENCE   3  (bases 1 to 9121)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9121)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-MAR-2010) OEB, Harvard University, 16 Divinity Ave., Cambridge, 
            MA 02138, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    2..565
                     /label=flank upstream of hpnH
                     /note="flank upstream of hpnH"
     misc_feature    595..1116
                     /label=flank downstream of hpnH
                     /note="flank downstream of hpnH"
     regulatory      701..710
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1740..2108)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(2141..2250)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     promoter        2887..2915
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             2963..4150
                     /label=TcR
                     /note="tetracycline efflux protein"
     CDS             complement(4334..4990)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             complement(5091..6509)
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
     promoter        complement(6510..6955)
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     rep_origin      complement(7367..7955)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8129..8986)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8987..9091)
                     /label=AmpR promoter