pAAV9-SBBANN-VBC vector (V009657)

LOCUS       V009657                 7954 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009657
VERSION     V009657
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7954)
  AUTHORS   Adachi K, Enoki T, Kawano Y, Veraz M, Nakai H.
  TITLE     Drawing a high-resolution functional map of adeno-associated virus
            capsid by massively parallel sequencing
  JOURNAL   Nat Commun 5, 3075 (2014)
   PUBMED   24435020
REFERENCE   2  (bases 1 to 7954)
  AUTHORS   Nakai H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2013) Department of Molecular and Medical
            Genetics, Oregon Health and Science University, 3181 SW Sam Jackson
            Park Road, Portland, OR 97239, USA
REFERENCE   3  (bases 1 to 7954)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7954)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun
            5, 3075 (2014)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (07-MAY-2013) Department of Molecular and Medical Genetics, Oregon
            Health and Science University, 3181 SW Sam Jackson Park Road,
            Portland, OR 97239, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   6..146
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     CDS             322..2184
                     /gene="Rep78"
                     /label="Protein Rep78"
                     /note="Protein Rep78 from Adeno-associated virus 2 (isolate
                     Srivastava/1982). Accession#: Q89268"
     CDS             4256..4267
                     /label="WELQut site"
                     /note="WELQut protease recognition and cleavage site"
     gap             4471..4482
                     /estimated_length=12
     gap             4503..4514
                     /estimated_length=12
     repeat_region   4670..4810
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     primer_bind     complement(4849..4865)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      5078..5533
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5815..5919
                     /label="AmpR promoter"
     CDS             5920..6777
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6951..7539
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    7827..7848
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7863..7893
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7901..7917
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7925..7941
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"